Table 4.
Summary of previous studies on BRAF mutation in colorectal carcinoma
| Study | Country | % with BRAF | BRAF mutation detected | Methods of mutation detection |
|---|---|---|---|---|
|
Li-Ling H, 2012 |
Taiwan |
1.1 |
2/182 |
High-resolution melting point (HRM) polymerase chain reaction (PCR) for BRAF V600E mutations |
|
Li H , 2011 |
China |
7 |
14/200 |
Sequenced by Pyrosequencer PyroMark ID system-Exon 15, V600E |
|
Liou J, 2011 |
China |
3.8 |
12/314 |
Sequencing of exons 11 and 15 |
|
Yokota T, 2011 |
Japan |
4.7 |
15/319 |
Cycleave PCR technique for V600E mutation |
|
Nakanishi R, 2012 |
Japan |
6.7 |
17/254 |
BRAF pyrokit pyrosequencing |
|
Ajay Goel, 2009 |
Israel |
18.7 |
24/128 |
Direct sequencing using the BigDye version 1.1 cycle-sequencing kit |
|
Rozek L, 2010 |
Israel |
5 |
65/1300 |
Direct sequencing of exon 15 of BRAF |
|
Price T, 2011 |
Australia |
10.6 |
33/315 |
High-resolution melting point (HRM) polymerase chain reaction (PCR) for BRAF V600E mutations |
|
Tran B, 2011 |
Australia |
11 |
57/524 |
Mutation-specific real-time polymerase chain reaction assay. |
|
Di Nicolantonio, 2008 |
Italy |
10 |
11/113 |
Automated sequencing by ABI PRISM 3730 exon15 |
|
Richman S, 2009 |
UK |
7.9 |
422/711 |
Pyrosequenced on a PyroMark ID system (Biotage AB) Codon 600 |
|
Roth A, 2009 |
Switzerland |
7.9 |
103/1307 |
Allelic discrimination assay on a 7500 real-time polymerase chain reaction for V600E |
|
Zlobec I, 2010 |
Switzerland |
12 |
45/374 |
BRAF (exon 15 codon 600) direct sequencing of single-stranded PCR products using the BigDyeVR Terminator v1.1 cycle sequencing kit |
|
Farina-Sarasqueta A, 2010 |
Netherlands |
19.8 |
59/297 |
V600E mutation on the BRAF gene by real-time PCRLight Cycler v2.0 (Roche) |
|
Saridaki Z, 2010 |
Greece |
8.3 |
12/144 |
Real-time PCR using the allelic discrimination method with ABI PRISM 7900 T Sequence Detection System |
|
Modest D, 2012 |
Germany |
11.6 |
17/146 |
Exons 11 and 15 of the BRAFpyro-sequencing |
|
Samowitz W, 2005 |
USA |
9.5 |
87/911 |
Sequencing in both directions |
|
Shaukat A,2010 |
USA |
21.8 |
36/165 |
BRAF V600E mutation -Mutector II BRAF/Ras mutation detection panel assay kit |
|
Kalady M,2012 |
USA |
12 |
56/475 |
Direct sequencing |
| Ogino S,2012 | USA | 15 | 11/75 | c.1799 T > A (p.V600E) mutation |