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. 2014 Jul 24;10(7):e1004467. doi: 10.1371/journal.pgen.1004467

Figure 4. MDRL can regulate miR-361 expression and activity.

Figure 4

A. LncRNAs expression levels upon treatment with A/R. Cardiomyocytes were untreated (control) or treated with A/R. LncRNAs expressed in heart in lncRNA array from Affymetrix company (http://www.noncode.org) were analyzed by qRT-PCR. *p<0.05 vs control. B. Knockdown of MDRL induces the decrease of MDRL expression levels. Cardiomyocytes were infected with adenoviral MDRL siRNA (MDRL-siRNA) and its scramble form (MDRL-sc). 24 h after infection MDRL levels were analyzed by real time PCR. *p<0.05 vs control. C. Knockdown of MDRL upregulates miR-361 expression levels. Cardiomyocytes were infected with adenoviral MDRL siRNA (MDRL-siRNA) and its scramble form (MDRL-sc). 24 h after infection miR-361 levels were analyzed by northern blot. D. Knockdown of MDRL induces miR-361 activity. Cardiomyocytes were infected with adenoviral MDRL-siRNA and its scramble, then transfected with miR-361 sensor. Luciferase activity was analyzed. *p<0.05 vs miR-361 sensor alone. E. Enforced expression of MDRL reduces the expression levels of miR-361. Cardiomyocytes were infected with adenoviral MDRL or β-gal. 24 h after infection MDRL levels were analyzed by real time PCR (low panel), and miR-361 levels were analyzed by northern blot (upper panel). F. MDRL reduces miR-361 activity. Cardiomyocytes were infected with adenoviral MDRL or β-gal, then transfected with miR-361 sensor. Luciferase activity was analyzed. *p<0.05 vs miR-361 sensor alone. G. MDRL acts as a sponge for miR-361 activity. Cardiomyocytes were infected with adenoviral miR-361, MDRL or β-gal, then transfected with miR-361 sensor. Luciferase activity was analyzed. *p<0.05.