Figure 3. TLO expression state is heritable.

(A) Ten Tloα12-GFP daughter cells were dissected away from their respective single mother cell. Both mother and daughter cells were grown independently for 18 hours and assayed for Tloα12-GFP expression by fluorescence microscopy of the resulting population. (B) Tloα12-GFP fluorescence of 50 cells for each population was collected by microscopy and the mean and standard deviation were plotted. Mother-daughter pairs were plotted together and generally show similar levels of mean expression between each pair, although more difference is evident in colonies 2 and 10 (C). The mean(ln) difference between cell expression data of mother-daughter pairs (red arrow) was tested against simulated datasets constructed from randomized mother-daughter affiliations(grey bars), and the association between mother-daughter pairs was highly significant.