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. 2014 Jul 24;10(7):e1004436. doi: 10.1371/journal.pgen.1004436

Figure 7. Sir2 contributes to TLO TAGEN.

Figure 7

(A) Transcript abundance measurements of six TLOs and two control genes were collected from either SIR2 or sir2Δ/Δ cells and in the presence or absence of the Sir-type HDAC inhibitor nicotinamide (NAM). Subtelomeric TLO expression plasticity specifically decreased when either treated with NAM or in the sir2Δ/Δ background but mean expression was not affected. Treatment of sir2Δ/Δ cells with NAM does not further decrease expression variability. (B) Fluorescence microscopy of GFP-tagged Tlos in either a SIR2 or sir2Δ/Δ background showed reduced cell-to-cell variation in a sir2Δ/Δ background. (C) Flow cytometry of GFP tagged Nup49, Tloα10, and Tloα12 also identified significantly reduced noise for both Tlos in the sir2Δ/Δ background. Fluorescence signal of Tloα10 was also increased in a SIR2 deletion strain. (D) Flow cytometry measured fluorescence signal of Nup49-GFP expressed at either the subtelomeric TLOα9 or internal NUP49 locus in both a SIR2 and sir2Δ/Δ background. Gene noise of subtelomeric Nup49-GFP decreased significantly in the sir2Δ/Δ background. * denotes p<0.05. ** denotes p<0.01.