Skip to main content
. 2014 Jul 24;10(7):e1004483. doi: 10.1371/journal.pgen.1004483

Figure 6. The protein level of Pol II and GTF subunits does not change after UVB treatment.

Figure 6

(A) Western blot assays were carried out to measure the levels of total Pol II at the indicated time points after UVB treatment by using the N-20 antibody. (B) The different IIA and IIO forms of Pol II were densitometrically quantified in each time points from four independent experiments and are represented in a bar chart, where red bars indicate Pol IIA and the blue bars indicate Pol IIO forms. In each lane Pol IIA+IIO signals are taken as 100%. Error bars represent +/− standard deviation of the four independent experiments. (C) The phosphorylation levels of Pol II Rpb1-CTD Ser2, Ser5 and Ser7 were analyzed by western blot by using the indicated antibodies, respectively. (D) The levels of the indicted GTF subunits such as TFIIB, TBP and TFIIH/CDK7 were also tested in the above prepared extracts, as indicated. Tubulin-α was used as a loading control.