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. 2014 Jul 24;10(7):e1004483. doi: 10.1371/journal.pgen.1004483

Figure 8. In the absence of CSB UVB does not inhibit Pol II and TFIIH binding at promoters of group A genes.

Figure 8

MCF7 cells were transfected with either scrambled siRNA or siRNAs targeting CSB (ERCC6) (see also Figure S4), 72 hours following siRNA transfection cells were treated with UVB, or not. ChIP was carried out 3 hours following UVB treatment of MCF7 cells as well as on non-treated cells. Q-PCRs were carried out to monitor Pol II (dark blue bars) (in A), and p62 (light blue bars) (in B) occupancy on the promoters of genes selected from group A (ubc, rplp1), group B (p21, wdr24) and on a negative control (intergenic) region in the presence or absence of CSB, as indicated. Control ChIPs (NoAb, red bars) was carried out with Sepharose G beads only. The occupancy values at the promoters are represented in input %. Error bars represent +/− standard deviations in two biological replicates.