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. 2014 Jul 24;9(7):e102186. doi: 10.1371/journal.pone.0102186

Figure 3. CDC42 signaling has a weaker influence on acute inflammation.

Figure 3

(A and B) Human endothelial cells were transduced with siRNA for CDC42, PAK2, RELA, IKKs, or control siRNA (siCont). Cells were treated with TNF-α (A) or LPS (B) at 60 hours after siRNA transduction and were harvested 24 hours later to examine the expression of pro-inflammatory genes by real-time PCR. (C) Knockdown efficiency of siRNAs for CDC42, PAK2, and NFκB signaling in endothelial cells at 60 hours after transduction. The graph shows expression of each gene in siRNA-treated cells relative to that in siCont-treated cells. Each experiment in Figure 3A–C was repeated three times, and the results were consistent. Representative results are shown.