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. 2014 Jul 24;9(7):e102566. doi: 10.1371/journal.pone.0102566

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© 2014 Sockolosky et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) SDS-PAGE analysis of purified mKate (lanes 1, 3) and mKate-IgGBP (lanes 2, 4) under reducing (lanes 1, 2) and non-reducing conditions (lanes 3, 4). 7.5 ug of protein was loaded in each lane. (b) Fluorescence emission spectra comparing equal molar concentrations (500 nM in D-PBS) of mKate and mKate-IgGBP. (c) Size exclusion chromatography standards and associated standard curve. The standards include thyroglobulin 670 kDa, gamma-globulin 158 kDa, ovalbumin 44 kDa, myoglobin 17 kDa, and vitamin B12 1.35 kDa. (d) Size exclusion chromatogram of mKate and mKate-IgGBP with retention time and calculated molecular weight. (e) MALDI-TOF analysis of mKate and mKate-IgGBP intact mass indicating a shift in molecular weight corresponding to the expected mass of the added flexible linker and IgGBP sequence.