Figure 1. Patterns of infiltration in GBM and determination of EGFR amplification by fluorescence in situ hybridization with the dual probe for EGFR (red color) and centromere of chromosome 7 (green color).

A. The cells tended to invade individually, giving a diffuse pattern (H&E, obj.x10). B. The infiltration was observed in groups of cells originating a nodular (or less-not diffuse) pattern showing a delimited front between the high density neoplastic cells and the peripheral nervous tissue (H&E, obj. x4). C. Tumor cells showing high-level amplification of the EGFR. D. Tumor cells with low-level amplification with other cells exhibiting a normal copy number of the EGFR gene. E. Cells without EGFR amplification.