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. 2014 Jul 24;9(7):e102907. doi: 10.1371/journal.pone.0102907

Figure 7. ERK independent mkp expression by NO regulates ERK activation.

Figure 7

(A) Hatched larvae were treated with 0.25 µM of dusp 1/6 I (+) in the absence or presence of TRIM (250 µM). Samples collected after 6 h of treatment were examined for ERK activation. Representative western blot analyzed with anti-P-ERK and anti-ERK antibodies. (B) Data of densitometric analyses of 3 independent experiments are reported as means ± SEM and assessed by unpaired t-test. Asterisk represents the significance respect to the control *P<0.05 (0.049), **P<0.01 (0.007), ***P<0.001 (0.0002). # represents the significance respect to the TRIM alone (P = 0.0008). (C) Gene regulation in response to MEK inhibitor U0126. Histogram shows the values of gene expression at 6 h treatment respect to the untreated larvae, followed by Real Time qPCR. Data are reported as a fold difference compared to the control (means ± SD). Values equal or greater than ±2 were considered significant. The experiments were repeated at least 3 times.