Table 1. Features of transcripts used for gene expression analysis: accession numbers, primer sequences, length of PCR amplified fragments and data on gene regulation and localization in larvae.
Gene Acronym | Accession Number | Gene Name | Gene Regulation | Localization | Primer sequences (5′-3′) | Fragment bp |
R27a | KYOTOGRAIL2005.209.6.1 | Ribosomal protein | reference | Ubiquitous | For-ATCCACCCTTCACCTTGTG | 156 |
Rev-GGAGATCTTGCCATTTTCA | ||||||
dhg | ci0100130315 | Dehydrogenase | down-regulated by ERK [11] | Ubiquitous | For-GTCACCGTTTCCTCTGAAGC | 299 |
Rev-GCGCCGTGTATTATGGTCTT | ||||||
ets | ci0100152617 | Ets-related transcription factor | up-regulated by ERK [11] | Papillae [11] | For-GACGAAGCATGTGTACCTGAC | 228 |
Rev-GCGGTTTTTCTGCACACCCC | ||||||
mkp1 | ci0100138796 | Dual specific MAPK phosphatase 1 | down-regulated by NO * | For-CCACTTTCCAGACCGATTTC | 401 | |
Rev-CCTCACAGGTCCACTCCATT | ||||||
mkp3 | ci0100140262 | Dual specific MAPK phosphatase 3 | down-regulated by NO * | For-CGATGTTGGCGTTGCTGTAC | 164 | |
Rev-GATGGACGGAGCATGAATGG | ||||||
mx | ci0100149359 | Matrix metalloprotease | up-regulated by ERK [11] | Papillae [11] | For-GATTCCCAGCTAGTATCCG | 263 |
Rev-CGTTCTTCTGCTTGGATTGT | ||||||
NOS | ci0100153759 | Nitric oxide synthase | Palps and tail [9] | For-AGAGTGAAAGCCTGTCGCATA | 150 | |
Rev-AACCAATGCGGTGGTTGTAG | ||||||
rev-erb | ci0100143297 | Steroid hormone receptor | down-regulated by NO * | Palps [33] | For-CAGATCCCATTGCCTGAGTTG | 152 |
Rev-CAGTCTATTTGGTCGTCGTCG | ||||||
srf | ci0100151810 | Serum responsive factor | For-CGTACTGTTGTTATGGCTG | 239 | ||
Rev-GTAGCTAACTCATGCGCC | ||||||
vwa1c | ci0100142643 | Von Willebrand factor | down-regulated by ERK [11] | Papillae [11] | For-GTATGTCAACATGTAGCATCG | 214 |
Rev-CACACGCTTCCTATAGACCTC |
*This work.