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. 2014 Jul 24;9(7):e102785. doi: 10.1371/journal.pone.0102785

Figure 3. Knockout of PRNP regulated phagosome maturation.

Figure 3

(A) qPCR analysis of the effects of PrPC on the expression of Rab5a, Rab7, and Eea1. Macrophages from PRNP−/− and wild-type (WT) mice were incubated with E. coli at 10 MOI for the indicated times. Total mRNA was isolated and reverse transcribed. The expression levels of the three markers were analyzed at each time point and are expressed as the fold change relative to the mRNA level in control cells exposed to PBS only. (B) Murine BMDMs were infected with EGFP-E. coli (green) at 10 MOI. Confocal fluorescence microscopy was used to image LAMP2 staining (red) in PRNP−/− and WT macrophages at 1 h. Recruitment was quantified (right panel). (C) Western blot analysis of LAMP2 expression after infection for 1 h. Data represent at least three independent experiments. *p≤0.05, **p≤0.01, ***p≤0.001.