Table 1. Energetic parameters of wild-type and Δhyd2 cells two hours following the induction of stationary phase.
Carbon Source | 5.5 mM Glycerol | 12.5 mM Acetate | ||
Strain | WT | Δhyd2 | WT | Δhyd2 |
Growth Yield (OD600) | 0.86±0.01 | 0.73±0.04 | 0.37±0.03 | 0.19±0.03 |
Growth Rate (h−1) | 0.19±0.04 | 0.17±0.01 | 0.17±0.01 | 0.07±0.01 |
External pH | 6.3±0.1 | 6.5±0.1 | 7.5±0.1 | 7.5±0.1 |
Internal pH | 7.3±0.2 | 7.1±0.1 | 7.1±0.1 | 7.2±0.2 |
Membrane Potential (mV) | −166±7 | −163±5 | −153±4 | −155±9 |
NAD+ Concentration (µM) | 1.6±0.4 | 1.6±0.1 | 0.90±0.14 | 0.95±0.12 |
NADH Concentration (µM) | 3.6±0.5 | 2.6±0.5 | 0.55±0.14 | 0.53±0.12 |
Error margins represent standard deviations from three biological replicates.