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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Oncogene. 2010 Nov 8;30(11):1341–1350. doi: 10.1038/onc.2010.513

Figure 4. Apoptosis induced by inhibition of MEK and PI3K/mTOR signaling in mouse astrocytes.

Figure 4

Cells were infected with viruses containing the genes indicated. After confirmation of gene expression, cells were treated in triplicate with either DMSO as a control, 0.5 μmol/L PD0325901 (901), 0.5 μmol/L NVP-BEZ235 (BEZ), or both (BEZ/901) in combination for 48 hours and apoptosis was quantitated using the Guava Nexin® assay using a Guava EasyCyte flow cytometer. Data were normalized to the controls and are represented as mean ± SEM.