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. 2014 Jul 24;9(7):e103305. doi: 10.1371/journal.pone.0103305

Figure 6. Anti-miR-21 restored gefitinib sensitivity of pc-9/GR in vivo.

Figure 6

(A) Fluorescent pictures of pc-9/GR cells transfected by inhibitors of control miRNAs (ctr) or miR-21. (B) Representative tumor engraftments in nude mice injected with pc-9/GR cells stably transfected with anti-Ctr, anti-21. 35 days after injection and following treatment with vehicle (0.1% tween 80) or gefitinib (200 mg/kg), mice were sacrificed. The images show one representative mouse in each category. (C) Comparison of engrafted tumors in nude mice injected with pc-9/GR cells stably transfected with inhibitors of control miRNAs (ctr) or miR-21. The images show representative tumors in each category. (D) Tumor growth curve upon implantation of pc-9/GR+anti-Ctr cells with vehicle or gefitinib. (E) Tumor growth curve upon implantation of pc-9/GR+anti-miR-21 cells with vehicle or gefitinib. (F) qRT-PCR showing miR-21 down-regulation in pc-9/GR xenografts infected by anti-miR-21 compared with pc-9/GR cells infected by anti-Ctr. *P<0.01. (G) Pten protein levels in xenograft tumors analyzed by western-blot.