Table 1. Correlation between FXYD3 expression and percent of methylation of individual CpGs.

	All subjects		Controls		T2D patients
CpGposition	r coeff	p value	r coeff	p value	r coeff	p value
–43	–0,7219	0,0016	–0,7143	0,0576	–0,9048	0,0046
–177	–0,7353	0,0012	–0,6667	0,0831	–0,9286	0,0022
–197	–0,5588	0,0244
–230	–0,6265	0,0094
–391	–0,5412	0,0304
–452	–0,5029	0,0471
–535	–0,5971	0,0146

DNA from control (n = 8) and T2D (n = 8) human islets were extracted from the same samples as used for the real-time PCR analysis. Then, the 32 CpGs of the FXYD3 promoter from −1 to −1200 were analyzed by pyrosequencing. Correlation analysis between methylation level and FXYD3 expression measured by quantitative RT-PCR was performed for each CpG using the non-parametric Pearson test. 7 CpG sites in the proximal FXYD3 promoter showed significant correlation between methylation level and FXYD3 expression. Within these 7 CpGs, 2 sites showed strongly significant correlation between methylation and FXYD3 mRNA expression only in the T2D group.