Figure 3. CMT1C LITAF mutations act in a dominant negative fashion.

BGMK cells were co-transfected with flag-tagged WT LITAF and either myc-tag WT LITAF, T49M and G112S. Twenty- four hours after transfection, the cells were probed with MitoTRACKER Red FM or LysoTRACKER DND-99 for 2 hrs then fixed and stained with anti-flag-antibodies (green), anti-myc-antibodies (red) and (A) the mitochondria was visualized using MitoTRACKER Red FM (purple) or (B) the late endosome/lysosomes were visualized using LysoTRACKER DND-99 (purple).