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. 2014 Jul 24;9(7):e103502. doi: 10.1371/journal.pone.0103502

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© 2014 Yamamoto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The length of B16F1 cell spheroids patterned in 10 cells/spheroid with 250 µm interval were image-analyzed by the green fluorescence after a 24 h culture with the HUVEC network. The plot represents the length of each B16F1 spheroid. The solid and dotted lines show the average length and the average length ±3× SD of B16F1 cell spheroids in 3D cell monoculture array.