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. 2014 Jul 24;9(7):e103563. doi: 10.1371/journal.pone.0103563

Figure 1. Purification and quantification of pro-mature BMP15.

Figure 1

A. Silver stained SDS-polyacrylamide gel of BMP15 samples. The pro-region appeared as two ∼40 KDa bands and the mature region as a ∼16 KDa band and as a ∼17 KDa band that has been reported to be O-linked glycosylated [68]. Lane 1: flow through. Lanes 2–3: wash 1–2. Lanes 4–7: eluted fractions 1–4. B. The processed mature region of pro-mature BMP15 was quantified by Western blotting [mab28 monoclonal antibody [42]] using the mature region of hBMP15 (R&D Systems) as a standard. Lanes 1–4: mature region of BMP15 (R&D Systems); 200, 100, 50 and 10 ng, respectively. Lanes 5–7: decreasing doses of the purified BMP15 pro-mature complex.