Table 3. Effect of supplementing COC during IVM with different forms of BMP15 (mature BMP15 and pro-mature BMP15), mature GDF9 at 100 ng/ml dose, with and without FSH on blastocyst quality.
Treatments | FSH | Inner cell mass | Trophectoderm | Total cell number (TCN) | ICM/TCN |
Control | − | 24.5±1.9 | 77.3±5.8 | 101.8±6.3 | 0.26±0.02 |
GDF9 mature | − | 27.4±2.0 | 73.8±4.6 | 101.3±5.2 | 0.28±0.02 |
BMP15 mature | − | 26.5±1.8 | 72.1±6.7 | 98.6±7.9 | 0.28±0.02 |
BMP15 pro-mature | − | 29.1±1.8 | 78.6±6.3 | 107.7±7.1 | 0.29±0.02 |
Control | + | 30.5±1.6 | 59.7±3.5 | 90.2±4.7 | 0.34±0.01 |
GDF9 mature | + | 28.7±1.9 | 69.7±5.0 | 98.4±6.0 | 0.30±0.01 |
BMP15 mature | + | 31.8±3.0 | 68.4±4.4 | 100.2±6.2 | 0.31±0.02 |
BMP15 pro-mature | + | 34.6±1.8 | 63.4±2.6 | 97.9±3.6 | 0.35±0.01 |
Data represents the mean ± SEM of 6 replicates. Mean blastocyst cell numbers following differential staining of 22–38 blastocysts.