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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Cell. 2014 Jun 19;158(1):171–184. doi: 10.1016/j.cell.2014.06.004

Figure 3. Structure-function analysis of YAP1.

Figure 3

(A) YAP1 domain structure and YAP1 mutants. (B) Effects of expressing YAP1 TEAD-defective mutants on the activity of a TEAD reporter in 293T cells. (C) Viability after KRAS suppression in HCTtetK cells expressing YAP1 mutants defective in TEAD activation. (D) Viability, in arbitrary luminescence units (ALU), after KRAS suppression in HCTtetK cells expressing a constitutively active TEAD2-VP16 fusion. (E) Effects of expressing the YAP1 mutants defective in transcriptional activation or nuclear localization in HCTtetK cells after KRAS suppression. **P-value <0.01. (B–E) Mean ± SD of 6 replicates of a representative experiment shown. (C–E) Viability of doxycycline treated relative to untreated samples displayed. See also Figure S3.