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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Cell. 2014 Jun 19;158(1):171–184. doi: 10.1016/j.cell.2014.06.004

Figure 5. YAP1 regulates the epithelial-mesenchymal transition (EMT).

Figure 5

(A) Enriched gene sets rescued by both YAP1 and KRAS. (B) qRT-PCR validation of EMT regulation by KRAS and YAP1 in HCT116 cells. Data represent mean +/− SD of four replicates relative to LacZ control. (C) Viability of doxycycline treated relative to untreated HCTtetK cells expressing Slug and Snail. (D) Effects of suppressing MYC after expressing FOS, Slug, and Snail in HCT116 cells. Viability of shMYC normalized to shLuciferase control in the presence of each indicated ORF. (E) Effect of Slug on the ability of YAP1 to rescue KRAS suppression. Viability of doxycycline-treated HCTtetK cells expressing YAP1 after expression of each indicated shRNA, normalized to media-treated shLuciferase control. (B–E) Mean ± SD of at least 3 replicates in a representative experiment shown. See also Figure S5.