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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Cell. 2014 Jun 19;158(1):171–184. doi: 10.1016/j.cell.2014.06.004

Figure 6. YAP1 and FOS interact at promoter regions to regulate EMT.

Figure 6

(A) Co-immunoprecipitation using control antibody or target-specific antibody for YAP1 and FOS in lysates from HCT116 cells expressing YAP1 and V5-tagged FOS. Binding of the reciprocal protein was assessed by immunoblotting. YAP1 indicated by arrowhead. *, IgG heavy and light chains. MW, molecular weight in kDa. (B) mRNA expression of Vimentin (VIM) and Slug after FOS suppression. Mean ± SD of four replicates relative to shLuciferase control in HCT116 cells shown. (C) Chromatin immunoprecipitation in HCT116 to assess YAP1 DNA binding at promoter regions of SLUG (SNAI2) and Vimentin (VIM). (D) Chromatin immunoprecipitation in HCT116 to assess YAP1 binding at SLUG promoter after FOS suppression. (C,D) Bars represent enrichment of promoter compared to 3′ region of each gene. Mean ± SD of 3 replicates shown. *P-value <0.05. See also Figure S6 and Table S4.