Figure 1.

Scheme for isolating DevS binding phages. Two wells of a 96 well plate were coated with DevS₂₀₁ protein and incubated overnight at 4°C. Next, the wells were blocked for 1 hr at 4°C. Phage library containing 2 × 10¹¹ pfu were added to the wells and incubated at room temperature. The unbound phages were removed by washing with TBS-Tween 20. The bound phages were eluted with 0.2 M glycine pH 2.2. The eluted phages were amplified and used as input for the next round of panning. The panning conditions were made stringent in each successive round of panning by decreasing the time of incubation of phage library with the protein and by increasing the concentration of Tween-20 in the washing steps.