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. 2014 Jul 21;14:195. doi: 10.1186/1471-2180-14-195

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Copyright © 2014 Kaur et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In vitro phosphotransfer assay. Reaction mixtures containing purified DevS ~ ³²P and GST- DevR full length/GST - DevR_N proteins were incubated at 25°C for 2 mins. Samples were analysed by 15% SDS-PAGE and autoradiography.