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. 2014 Jul 10;48(3):247–256. doi: 10.2478/raon-2014-0025

FIGURE 3.

FIGURE 3.

Effects of hindlimb irradiation on gastrocnemius muscle oxidative stress and protein turnover. A Upper left. Representative immunoblot of 4HNE protein expression. Lower left. Quantification of 4HNE protein expression. Upper right. Representative immunoblot of ubiquitin protein expression. Lower right. Quantification of ubiquitin protein expression. B Upper. Representative immunoblot of phosphorylated and total FOXO3A, and total Atrogin-1, LC3B and GAPDH protein expression. Lower. Quantification of phosphorylated and total FOXO3A, and total Atrogin-1, LC3B and GAPDH proteins. Values are normalized to control and are presented as means ± standard error. Statistical significance was set at P<.05. * = statistically different from control; 4-HNE = 4-hydroxynonenal; μg = microgram; Gy = gray.