Figure 6. Glucose uptake is reduced by visfatin inhibition in 3T3-L1 adipocytes. (A) Adipocytes were incubated with or without TNFα (15 ng/mL) and in the presence of FK866 at 1 nM for 24 h. Cells were serum-starved for 1 h before a 30 min stimulation with insulin (0 and 170 nM). 2-deoxy-D-[³H]glucose uptake was measured as described in Materials and Methods. The uptake measurements were performed in triplicates and normalized to protein concentrations. Results (means ± SEM) are expressed as percentage of maximum uptake. (B) Akt phosphorylation is reduced by visfatin inhibition in differentiated 3T3-L1 cells. Adipocytes were incubated with or without TNFα (15 ng/mL) and in the presence of FK866 at 1 nM for 24 h. Total cell lysates (40 μg) were subjected to SDS-PAGE and immunoblotted with phospho-AKT or AKT antibodies. The western blot is representative of three independent experiments.