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Making of Cre recombinase-expressing 293 cells. A) Functional assay of Cre activity. In the plox-DsRed-lox-EGFP plasmid-transfected cells, Cre-lox reaction deletes the DsRed gene and expresses the EGFP gene. B) Expression of Cre recombinase mRNA in a selected 293-Cre clone. Total RNA was isolated from 293-Cre2 cells and subjected to RT-PCR analysis.
