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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Exp Zool B Mol Dev Evol. 2014 Jan 24;322(6):438–463. doi: 10.1002/jez.b.22558

Figure 13. Annotation of the scaffolds encoding coelacanth interleukin genes.

Figure 13

Genes encoding interleukins were identified from the coelacanth annotated assembly at the Ensembl. Coding sequences were retrieved from their corresponding scaffolds and imported into Vector NTI sequence analysis software (Invitrogen). GENESCAN (Burge and Karlin 1997) and BLASTX programs were used, respectively, to predict the exon-intron boundaries and determine amino acid alignments to other vertebrates.