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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Exp Zool B Mol Dev Evol. 2014 Jan 24;322(6):438–463. doi: 10.1002/jez.b.22558

Figure 5. IgW transcript validation in coelacanth.

Figure 5

IgW1 and IgW2 heavy chain transcripts were identified in the L. menadoensis RNAseq combined assembly, despite the dataset being generated from non-lymphoid (liver and testes) sources. (A) A rearranged IgW1 heavy chain transcript that includes a partial VD region, a known J-segment and seven constant domains (CH1 to CH7) followed by a secretory tail (upper panel). Another IgW1 heavy chain transcript that lacks the variable region and most of the constant region but contains a CH5 domain spliced to transmembrane domains followed by its 3’ UTR, suggests it derives from a membrane isoform of IgW. (B) The IgW2 heavy chain transcript lacks a variable domain but possess an intact constant region (7 domains) in which its secretory tail was attached at the 3’ end of CH7 domain. Three other constant region transcripts were identified in the muscle transcriptome of L. chalumnae (not shown).