FIGURE 3.
PAGE of the SGT purification process. A, IEF-PAGE separation of proteins partially purified by native PAGE. One hundred percent corresponds to 11.1 units (nmol/min/mg protein) in the SGT assay with protein extracted from gel piece 9. B, SDS-PAGE of protein extracted from gel pieces 5–11 shown in A. Gel piece 9 showed the highest SGT activity, and an 80–90-kDa protein indicated by the box was recovered from the gel and then analyzed by MS/MS.