Skip to main content
. 2014 Jun 9;289(30):20405–20420. doi: 10.1074/jbc.M114.553933

FIGURE 3.

FIGURE 3.

PAGE of the SGT purification process. A, IEF-PAGE separation of proteins partially purified by native PAGE. One hundred percent corresponds to 11.1 units (nmol/min/mg protein) in the SGT assay with protein extracted from gel piece 9. B, SDS-PAGE of protein extracted from gel pieces 5–11 shown in A. Gel piece 9 showed the highest SGT activity, and an 80–90-kDa protein indicated by the box was recovered from the gel and then analyzed by MS/MS.