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. 2014 Jun 9;289(30):20405–20420. doi: 10.1074/jbc.M114.553933

FIGURE 6.

FIGURE 6.

Characterization of SGT activity of recombinant CrSGT1 (CD+UR) toward AtEXT peptide (n = 3). A, requirement for nucleotide sugars. Assay mixtures were incubated with or without 5 mm various nucleotide sugars. 100% corresponds to 6.4 × 102 unit (nmol/min/mg protein) with UDP-galactose. B, requirement for divalent metal cation. Assay mixtures were incubated with or without various divalent metals. 100% corresponds to 6.5 × 102 unit (nmol/min/mg protein) with manganese. C, optimal pH. Buffers for assay mixtures were 0.1 m sodium acetate (diamonds), 0.1 m MES-NaOH (squares), 0.1 m MOPS-NaOH (triangles), and 0.1 m Tris-HCl (circles). 100% corresponds to 6.0 × 102 unit (nmol/min/mg protein) at pH 6.0 of 0.1 m MES-NaOH. D, optimal temperature. Assay mixtures were incubated at 20–60 °C. 100% corresponds to 6.1 × 102 unit (nmol/min/mg protein) at 30 °C.