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. 2014 Jun 4;289(30):20559–20569. doi: 10.1074/jbc.M114.573006

FIGURE 5.

FIGURE 5.

Transcriptional repression and direct binding of YafQ-(DinJ)2-YafQ at the dinJ promoter. A, wild-type promoter region of dinJ with each IR of operator 1 and 2, the −35 and −10 promoter regions, the ribosome-binding site (RBS), and the LexA consensus box indicated. Nucleotides depicted in red were mutated to test for transcriptional repression by YafQ-(DinJ)2-YafQ. B, β-galactosidase activity assays testing the repression at the dinJ operator by wild-type YafQ-(DinJ)2-YafQ. Error bars represent mean ± S.E. of experiments performed in triplicate. C, EMSAs with increasing concentrations of wild-type YafQ-(DinJ)2-YafQ binding to either wild-type or IR1′-2–2′ mutated dinJ promoter region.