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. 2014 Jun 3;289(30):20583–20593. doi: 10.1074/jbc.M114.569251

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Robust simulation of BDKDC activity in mouse tissues with decreased plasma BCAA concentrations. A, BCKDC activity in heart, muscle, kidney, and liver from wild-type C57BL/6J mice treated with vehicle (V) or BT2 (T) for 1 week. Shaded bars represent the mean of BCKDC activities in tissues from an individual mouse (n = 4); *, p < 0.05; ****, p < 0.0001. B, relative protein levels of phosphorylated E1 (pE1) and total E1 (E1) in tissues from vehicle- and BT2-treated mice. GAPDH served as a loading control. The amounts of tissue homogenate applied were 15 μg for liver, 30 μg for heart and kidney, and 60 μg for muscle. C, decreased plasma BCAA concentrations in BT2-treated mice compared with vehicle-treated (n = 4). Leu + Ile, sum of the two amino acids that are indistinguishable in LC-MS/MS. Error bars, S.D.