Protein and mRNA levels of BDK in kidneys and heart from vehicle- and BT2-treated wild-type mice.
A, Western blots (top) of kidney and heart homogenates from vehicle-treated (V) and BT2-treated (T) mice with GAPDH as loading controls. The quantification of band densities shows that BDK protein levels in BT2-treated kidney and heart are reduced to 39 and 24%, respectively, compared with the vehicle-treated ones (bottom). B, BDK mRNA levels determined by quantitative PCR in kidneys from BT2-treated mice relative to the vehicle-treated ones (set as 1.0). The GAPDH mRNA level was used as an internal control. Error bars, S.D.