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. 2014 Jun 4;289(30):20706–20716. doi: 10.1074/jbc.M114.547828

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Translational induction of AIRAP. A, immunoblot of AIRAP and p97 (used as a loading control) in mouse embryonic fibroblasts (MEFs) pretreated with arsenite (25 μm) for the indicated time course. AIRAP mRNA levels from the same cells were quantified and normalized to a housekeeping gene using qPCR, and fold induction is represented in the right panel. Quantitation and statistical evaluations were obtained from three independent experiments. Comparison of Time 0 with Time 90 showed a statistical significance of t test p < 0.001. B, left panel, metabolic labeling performed in MEFs pretreated with arsenite for the indicated time course. Cell lysates were resolved by SDS-PAGE, and the autoradiogram is presented. Right panel, polysome profiles from untreated and arsenite-treated cells showing the accumulation of ribosomal subunits and monosomes in the arsenite-treated cells.