FIGURE 3.

AIRAP translation regulation is mediated by means of leaky scanning. A, diagram presentation of the reporters used. B, autoradiogram of radiolabeled proteins after a brief labeling pulse of untreated and arsenite-pretreated 293T cells. Cells were transfected with the indicated reporter and subjected to a GFP IP resolved by SDS-PAGE. Untreated 5′ AIRAP WT reporter quantities were set as 1, and relative quantities are shown below. All quantifications were normalized to GFP mRNA transcript levels. Quantitation and statistical evaluations are present from three independent experiments.