FIGURE 2.

CHD5 increases accessibility of nucleosomal DNA at two sites without significant changes in nucleosome mass. Chromatin was incubated with CHD5 in the absence (A) or presence (B) of ATP, digested with MNase, and loaded onto a 10% to 40% linear glycerol gradient. Following sedimentation, fractions were collected, and the DNA in each fraction was purified and resolved by agarose gel electrophoresis. To better visualize the DNA fragments, peak fractions were resolved by PAGE and visualized by SYBR green staining. DNA fragments corresponding to mono-, di-, and trinucleosomes are indicated. DNA fragments derived from mononucleosomes digested at one internal site are indicated with the open arrowhead and those digested at two sites are indicated with the asterisk. DNA fragments from dinucleosomes digested at one internal site are indicated with the solid arrowhead, and fragments from dinucleosomes digested at two sites are indicated with the double asterisk.