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20 S proteasome degrades PICT1. Immunopurified Myc-PICT1 (A) and Myc-EGFP (B) were subjected to 20 S proteasome-mediated in vitro degradation assay. After incubation for the indicated times, Myc-tagged proteins and mouse IgG heavy chain (IgG-H) were detected by immunoblot analysis as described under “Experimental Procedures.”
