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Nucleolar stress does not alter intracellular localization of PICT1. H1299 cells were treated for 6 h with 1 μm epoxomicin (Epoxo), 5 nm ActD, 1 mm FUrd, alone or together as indicated. Intracellular localization of endogenous PICT1 and nucleolin was analyzed by immunofluorescence assay as described under “Experimental Procedures.”
