FIGURE 5.

Protein expression and phosphorylation of metabolic enzymes. A, phosphorylation of PKM2 at Tyr-105 is decreased 24 h after EGFR-TKI addition. EGFR inhibition in the TKI-sensitive HCC827 and PC9 cells did not induce significant reduction of phospho-PKM2 at either 2 or 6 h post-treatment. B, Western blotting showing proteins related with oncogenic signaling, GLS, GYS, GLUTs, HKII, PDHA1, and phospho-PDHA1. Total protein lysates were isolated from cells treated with gefitinib or erlotinib for 6 h. Equivalent amounts of proteins from whole-cell lysates were subjected to Western blot analysis to detect the indicated proteins. β-Actin was used as a loading control.