FIGURE 5.

H₂S has no effect on AR nuclear translocation and phosphorylation. A, H₂S did not affect cellular localization of AR. Seventy-two hours after LNCaP cells were incubated with R1881 (10 nm) with or without NaHS (30 μm), the cells were observed for GFP signal under an inverted Olympus IX70 microscope. B, H₂S had no effect on AR nuclear localization. Forty-eight hours after LNCaP cells were incubated with R1881 (10 nm) with or without NaHS (30 μm), the cells were collected to extract nuclear and cytosolic protein. C, H₂S did not change the binding of AR with HSPs. Forty-eight hours after LNCaP cells were incubated with R1881 (10 nm) with or without NaHS (30 μm), the cells were collected for immunoprecipitation (IP) assay. WB: Western blotting. D, H₂S had little effect on AR phosphorylation (phos-AR). LNCaP cells were incubated with R1881 (10 nm) with or without NaHS (30 μm) for 2 h. The experiments were repeated at least three times.