FIGURE 1.

Identification of an alternative splice form of Sec24c. A, schematic of the two Sec24c splice forms observed in mice. Arrows indicate primers used to detect the presence of the alternative exon (6*). Shaded regions in exons 4 and 5 represent exonic sequence present in Sec24c-2 that is absent from another RefSeq splice variant annotated as containing exon 6* (NM_001168273.1), which was not detected in our RT-PCR. B, protein sequence alignment beginning at Thr-306 of SEC24C-1 and SEC24C-2 showing the additional 23 amino acids encoded by exon 6*. C, RT-PCR using primers V and W, which detects a 249-bp product from Sec24c-2 and a 180-bp product from Sec24c-1.