FIGURE 6.

Ubiquitous transgenic expression of Sec24c and Sec24d. A, diagram of the pCAG3zS-Sec24c or -Sec24d transgenic construct, adapted from Ref. 38, including transcription start site, initial ATG, and polyadenylation signal. CMV IE = cytomegalovirus immediate-early enhancer; UTR = untranslated region; SV40 poly(A) = simian virus 40 polyadenylation signal. Primers 24c-F, 24c-R, 24d-F, and 24d-R were used for RT-PCR of transgenes expressing Sec24c or Sec24d, respectively. B, transgene expression in a panel of tissues for founder lines 24c-line1, 24d-line1, 24d-line2, and 24d-line3. Heart and brain RT samples were carried out in the absence of reverse transcriptase.