FIGURE 1.

The cytoplasmic C terminus of NHE5 binds to the AMPKα2 catalytic subunit in vitro. A, schematic representation of the predicted transmembrane organization of NHE5. B, purified GST and GST-NHE5 fusion proteins encoding peptide segments that span the cytoplasmic C terminus of NHE5 were incubated with in vitro translated full-length ³⁵S-labeled AMPKα2 protein. The proteins in the reaction solution were resolved by SDS-PAGE. Radioactivity was detected using a PhosphorImager and protein loading was visualized by staining with Coomassie Blue (CB) dye. Data are representative of at least three independent experiments.