FIGURE 9.

Activation of NHE5 in response to acute metabolic stress is dependent on AMPK. AP-1/NHE5_HA3 cells were treated over a 60-min period either separately or together with AA (1 μg/ml) to activate AMPK and the specific AMPK inhibitor CC (20 μm). NHE5 activity was measured as EIPA-inhibitable ²²Na⁺ influx (nmol/min/mg protein) and normalized as a percentage of the basal rate at the start of the experiment (0 min). Data were generated from quadruplicate samples in each of three independent experiments and presented as the mean ± S.E.