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Deleting GCN2 does not rescue the translation defect in eEF1A-Ura3p mutants. Total translation was monitored in exponentially growing wild-type (A) and eEF1A-Ura3p mutant strains (B, C, and D) with and without GCN2 as described in the legend to Fig. 2 by measuring [35S]methionine incorporation for the indicated times. [35S]Methionine incorporation is expressed as cpm/A600 unit of cells.
