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. 2014 May 29;289(30):20960–20969. doi: 10.1074/jbc.M114.573642

FIGURE 4.

FIGURE 4.

A. niger Bgl1 is a homodimer. Native PAGE of A. niger BglI is shown. 30 μg of BglI was run on a 2–12% Native PAGE (Invitrogen) and stained with SimplyBlue protein stain. The protein molecular mass aligns as a dimer, with no detectable band for monomeric species. There is, however, a band running at a higher molecular mass that may be a tetramer or higher order oligomeric species.