FIGURE 3.

DEER analysis of the movement of the C-tail and arrestin-2 domains. Fits to the free (black) and PR*-bound (red) background-corrected dipolar evolution data (gray dots) are plotted on the left for the C-tail (A and B) and interdomain (C–E) mutants to illustrate the data quality and support the distance distribution data. The corresponding distance distributions are shown on the right as overlays for the free (black) and PR*-bound (red) states. F, the free state crystal structure (Protein Data Bank entry 1G4M) of arrestin-2 is labeled with each pair of double mutants (red spheres). Measured distances are shown as dotted lines.