FIGURE 1.

A2bAR expression and function in SVCs. SVCs were isolated from mouse inguinal adipose tissue and, upon confluence, induced to differentiate to adipocytes as described under “Experimental Procedures.” A, cAMP levels were determined following 10-min treatment of SVCs with induction mixture and vehicle (DMSO), 1 μm BAY 60-6583 (BAY), or 10 μm 5′-(N-ethylcarboxamido) adenosine (NECA). *, p < 0.05; **, p < 0.01 compared with vehicle (n = 4). B, the expression of A2aAR and A2bAR was determined by quantitative PCR at various time points during differentiation. Relative mRNA expression was determined using the ΔΔCT method and normalized to 18 S rRNA. *, p < 0.05; ***, p < 0.001 compared with the 0 h time point (n = 4). Data are mean ± S.E. Analyses were performed by one- or two-way ANOVA with Bonferroni multiple comparison post hoc test.